1、一種耐高溫棉花雜交種的選育方法 

申請（專利）號：200710086690.7摘要：一種(zhong)(zhong)耐高(gao)溫棉花雜(za)(za)交(jiao)(jiao)(jiao)種(zhong)(zhong)的(de)選(xuan)(xuan)育方(fang)法(fa)，屬農作物抗逆育種(zhong)(zhong)領域(yu)(yu)。該選(xuan)(xuan)育方(fang)法(fa)如(ru)下：1）選(xuan)(xuan)擇親本，進(jin)(jin)行雜(za)(za)交(jiao)(jiao)(jiao)，獲(huo)(huo)得棉花雜(za)(za)交(jiao)(jiao)(jiao)種(zhong)(zhong)F1；2）按(an)隨機區(qu)組(zu)試驗設計種(zhong)(zhong)植(zhi)棉花雜(za)(za)交(jiao)(jiao)(jiao)種(zhong)(zhong)F1，在田間調查單(dan)株結鈴數和(he)成鈴率(lv)；3）室內離體花粉(fen)培養測定(ding)棉花雜(za)(za)交(jiao)(jiao)(jiao)種(zhong)(zhong)F1的(de)花粉(fen)萌發率(lv)和(he)花粉(fen)管生長長度；4）采用主成分(fen)(fen)分(fen)(fen)析(xi)(xi)方(fang)法(fa)進(jin)(jin)行篩選(xuan)(xuan)，根據步驟2）和(he)3）獲(huo)(huo)得的(de)結果進(jin)(jin)行主成分(fen)(fen)分(fen)(fen)析(xi)(xi)，然后以(yi)主成分(fen)(fen)分(fen)(fen)析(xi)(xi)后的(de)第(di)一和(he)第(di)二(er)主分(fen)(fen)量(liang)數值對所測定(ding)的(de)棉花雜(za)(za)交(jiao)(jiao)(jiao)種(zhong)(zhong)F1進(jin)(jin)行篩選(xuan)(xuan)。用本發明(ming)方(fang)法(fa)培育出(chu)結鈴性強、高(gao)產和(he)適應性強的(de)耐高(gao)溫棉花雜(za)(za)交(jiao)(jiao)(jiao)種(zhong)(zhong)，解決(jue)例如(ru)我國長江流域(yu)(yu)雜(za)(za)交(jiao)(jiao)(jiao)棉花種(zhong)(zhong)植(zhi)區(qu)域(yu)(yu)在7月至8月份期(qi)間常出(chu)現(xian)高(gao)溫逆境(jing)而導致蕾(lei)鈴脫落，并嚴重影響皮棉產量(liang)和(he)品質的(de)問題(ti)。 

2、超聲波輔助農桿菌轉化棉花胚芽的方法 

申請（專利）號：200710063650.0 

摘要：本發明屬于(yu)棉(mian)(mian)花(hua)(hua)轉(zhuan)基(ji)(ji)因(yin)(yin)(yin)技術領域，公開了(le)超聲(sheng)波輔(fu)助(zhu)農(nong)桿菌(jun)(jun)轉(zhuan)化棉(mian)(mian)花(hua)(hua)胚(pei)(pei)芽的(de)培(pei)(pei)(pei)育轉(zhuan)基(ji)(ji)因(yin)(yin)(yin)棉(mian)(mian)花(hua)(hua)的(de)新方(fang)法。包(bao)括(kuo)：將(jiang)(jiang)剝(bo)掉(diao)種皮消毒后(hou)棉(mian)(mian)種接種到(dao)(dao)(dao)(dao)無菌(jun)(jun)苗萌(meng)發培(pei)(pei)(pei)養(yang)基(ji)(ji)上，將(jiang)(jiang)剛萌(meng)發兩片子葉剝(bo)離，置潤濕的(de)無菌(jun)(jun)濾紙上，將(jiang)(jiang)10ml0.5OD600mm的(de)農(nong)桿菌(jun)(jun)菌(jun)(jun)液(ye)加入到(dao)(dao)(dao)(dao)塑料(liao)離心管(guan)或(huo)(huo)玻璃小三角瓶(ping)中(zhong)并(bing)將(jiang)(jiang)剝(bo)好的(de)胚(pei)(pei)芽加到(dao)(dao)(dao)(dao)農(nong)桿菌(jun)(jun)液(ye)中(zhong)，將(jiang)(jiang)離心管(guan)或(huo)(huo)者小三角瓶(ping)放在(zai)超聲(sheng)波清洗器中(zhong)的(de)中(zhong)央，用100～150W的(de)超聲(sheng)波處理1-5min，將(jiang)(jiang)處理過的(de)胚(pei)(pei)芽轉(zhuan)移(yi)至共培(pei)(pei)(pei)養(yang)培(pei)(pei)(pei)養(yang)基(ji)(ji)培(pei)(pei)(pei)養(yang)，再(zai)用含頭孢霉(mei)素的(de)無菌(jun)(jun)水(shui)沖洗，吸干胚(pei)(pei)芽表(biao)面水(shui)分，轉(zhuan)移(yi)至恢復培(pei)(pei)(pei)養(yang)基(ji)(ji)上，在(zai)光照下(xia)(xia)恢復培(pei)(pei)(pei)養(yang)得(de)(de)到(dao)(dao)(dao)(dao)幼(you)芽，將(jiang)(jiang)其轉(zhuan)到(dao)(dao)(dao)(dao)選擇培(pei)(pei)(pei)養(yang)基(ji)(ji)繼(ji)代，得(de)(de)到(dao)(dao)(dao)(dao)抗性芽，將(jiang)(jiang)其切(qie)下(xia)(xia)轉(zhuan)移(yi)至生根(gen)培(pei)(pei)(pei)養(yang)基(ji)(ji)上誘導生根(gen)或(huo)(huo)嫁接在(zai)棉(mian)(mian)花(hua)(hua)砧木(mu)上，得(de)(de)到(dao)(dao)(dao)(dao)再(zai)生轉(zhuan)基(ji)(ji)因(yin)(yin)(yin)植(zhi)(zhi)株。本發明將(jiang)(jiang)GFP（綠(lv)色熒(ying)光蛋白）基(ji)(ji)因(yin)(yin)(yin)在(zai)胚(pei)(pei)芽的(de)瞬時表(biao)達率(lv)提高100多倍；首次實(shi)現(xian)了(le)海島棉(mian)(mian)（長絨棉(mian)(mian)）的(de)轉(zhuan)基(ji)(ji)因(yin)(yin)(yin)植(zhi)(zhi)株；獲得(de)(de)了(le)一批轉(zhuan)抗蟲基(ji)(ji)因(yin)(yin)(yin)的(de)棉(mian)(mian)花(hua)(hua)植(zhi)(zhi)株。分子及田間實(shi)驗檢測表(biao)明，外源基(ji)(ji)因(yin)(yin)(yin)已經穩定轉(zhuan)入到(dao)(dao)(dao)(dao)受(shou)體(ti)棉(mian)(mian)花(hua)(hua)基(ji)(ji)因(yin)(yin)(yin)組(zu)中(zhong)并(bing)能穩定表(biao)達和遺傳(chuan)。 

3、光敏核不育棉花基因資源及其應用技術 

申請(qing)（專利）號：200610124597.6 

摘要：本發明光(guang)敏核(he)(he)不(bu)(bu)育(yu)棉花基因(yin)資(zi)源(yuan)(yuan)及其應用(yong)技術，在特殊(shu)生(sheng)態環境下：在夜間燈光(guang)照(zhao)(zhao)射不(bu)(bu)止，長期石(shi)油(you)油(you)泥油(you)氣(qi)的污染農田中，將多(duo)個棉花品種(zhong)(zhong)(zhong)進行種(zhong)(zhong)(zhong)植，誘導出(chu)一變異單株(zhu)，只結有2個晚(wan)秋桃(tao)，對(dui)其留后，經過多(duo)年持續(xu)不(bu)(bu)斷篩(shai)選(xuan)，獲得光(guang)敏核(he)(he)不(bu)(bu)育(yu)棉花基因(yin)資(zi)源(yuan)(yuan)，給棉花雜交(jiao)制種(zhong)(zhong)(zhong)開辟了新的育(yu)種(zhong)(zhong)(zhong)方法(fa)和(he)利用(yong)途徑(jing)，其特性為日(ri)照(zhao)(zhao)時(shi)數≥13.6nr，表(biao)現雄性不(bu)(bu)育(yu)，日(ri)照(zhao)(zhao)時(shi)數≥13.2nr表(biao)現雄性可育(yu)，不(bu)(bu)育(yu)期內可用(yong)作母本，不(bu)(bu)需去雄直接(jie)授粉(fen)雜交(jiao)制種(zhong)(zhong)(zhong)，可育(yu)期內可自交(jiao)繁殖種(zhong)(zhong)(zhong)子，適(shi)宜我國大部分棉花制種(zhong)(zhong)(zhong)基地配(pei)組使用(yong)。 

4、棉花品種人工網室抗棉蚜鑒定方法 

申請(qing)（專利）號：200610037612.3 

摘要(yao)：本發明(ming)(ming)涉及(ji)一(yi)種以人(ren)工(gong)(gong)網(wang)室為鑒(jian)定(ding)場所的棉(mian)(mian)花品(pin)(pin)種抗(kang)棉(mian)(mian)蚜(ya)(ya)(ya)(ya)鑒(jian)定(ding)的改良(liang)方法(fa)。該(gai)方法(fa)具體(ti)操作步驟為：根據(ju)鑒(jian)定(ding)規模建立人(ren)工(gong)(gong)網(wang)室；冬(dong)季深(shen)翻土壤(rang)，利(li)用低溫(wen)凍害殺死蟲卵；開春(chun)后在人(ren)工(gong)(gong)網(wang)室內播種常(chang)規棉(mian)(mian)品(pin)(pin)種，建立棉(mian)(mian)蚜(ya)(ya)(ya)(ya)繁(fan)殖行(xing)；當棉(mian)(mian)苗(miao)普遍達到3葉期(qi)時，將供試材料分別(bie)栽入人(ren)工(gong)(gong)網(wang)室棉(mian)(mian)蚜(ya)(ya)(ya)(ya)繁(fan)殖行(xing)之間，進(jin)行(xing)接蚜(ya)(ya)(ya)(ya)處(chu)理；分別(bie)于苗(miao)蚜(ya)(ya)(ya)(ya)期(qi)、伏蚜(ya)(ya)(ya)(ya)期(qi)及(ji)秋(qiu)蚜(ya)(ya)(ya)(ya)期(qi)調查供試品(pin)(pin)種的蚜(ya)(ya)(ya)(ya)害級(ji)別(bie)；以常(chang)規品(pin)(pin)種為對照，計算蚜(ya)(ya)(ya)(ya)害指(zhi)數及(ji)其(qi)比對照減(jian)退(tui)率(lv)；建立棉(mian)(mian)花品(pin)(pin)種對棉(mian)(mian)蚜(ya)(ya)(ya)(ya)的抗(kang)性分級(ji)標準(zhun)，根據(ju)各供試品(pin)(pin)種的蚜(ya)(ya)(ya)(ya)害指(zhi)數比對照減(jian)退(tui)率(lv)，確定(ding)其(qi)對棉(mian)(mian)蚜(ya)(ya)(ya)(ya)的抗(kang)性水(shui)平。本發明(ming)(ming)能夠顯著地提高棉(mian)(mian)花品(pin)(pin)種抗(kang)棉(mian)(mian)蚜(ya)(ya)(ya)(ya)鑒(jian)定(ding)的準(zhun)確率(lv)和(he)可(ke)靠性。 

5、棉花的二個DREB類轉錄因子及其編碼基因與應用 

申請(qing)（專利(li)）號：200610012084.6 

摘要(yao)：本發(fa)明公開了一(yi)個棉花的(de)(de)(de)(de)DREB類(lei)轉(zhuan)(zhuan)錄因子及其(qi)編(bian)碼(ma)基(ji)因與應(ying)用(yong)。其(qi)目的(de)(de)(de)(de)是提供一(yi)個來源于棉花的(de)(de)(de)(de)DREB類(lei)轉(zhuan)(zhuan)錄因子及其(qi)編(bian)碼(ma)基(ji)因與其(qi)在(zai)培育抗(kang)逆(ni)性提高的(de)(de)(de)(de)植物(wu)中(zhong)的(de)(de)(de)(de)應(ying)用(yong)。該DRIB類(lei)轉(zhuan)(zhuan)錄因子是下述氨基(ji)酸殘(can)(can)基(ji)序列(lie)之一(yi)：1）序列(lie)表中(zhong)的(de)(de)(de)(de)SEQID№：1；2）將序列(lie)表中(zhong)SEQID№：1的(de)(de)(de)(de)氨基(ji)酸殘(can)(can)基(ji)序列(lie)經(jing)過一(yi)至十(shi)個氨基(ji)酸殘(can)(can)基(ji)的(de)(de)(de)(de)取代、缺失或添(tian)加且具有轉(zhuan)(zhuan)錄激(ji)活(huo)功能(neng)的(de)(de)(de)(de)調控植物(wu)抗(kang)逆(ni)性的(de)(de)(de)(de)蛋白質。本發(fa)明可應(ying)用(yong)于植物(wu)抗(kang)性的(de)(de)(de)(de)基(ji)因工(gong)程(cheng)改(gai)良(liang)中(zhong)，具有較高的(de)(de)(de)(de)實際應(ying)用(yong)價值。 

6、以葵花為砧木的棉花稼接栽培方法 

申請（專(zhuan)利）號：200510094541.6 

摘要：以(yi)(yi)(yi)葵花(hua)(hua)(hua)(hua)(hua)為砧(zhen)(zhen)木的(de)棉(mian)(mian)花(hua)(hua)(hua)(hua)(hua)稼接栽培方(fang)法(fa)，其特(te)征是在(zai)(zai)清(qing)明至(zhi)(zhi)立夏(xia)期間，先育(yu)(yu)葵花(hua)(hua)(hua)(hua)(hua)苗；待(dai)葵花(hua)(hua)(hua)(hua)(hua)苗長(chang)出4～6片葉時，進行棉(mian)(mian)籽(zi)(zi)催芽；以(yi)(yi)(yi)葵花(hua)(hua)(hua)(hua)(hua)為砧(zhen)(zhen)術，將(jiang)葵花(hua)(hua)(hua)(hua)(hua)在(zai)(zai)地表以(yi)(yi)(yi)上、底部(bu)(bu)葉片以(yi)(yi)(yi)下的(de)莖(jing)(jing)部(bu)(bu)切口(kou)，切口(kou)深(shen)至(zhi)(zhi)木質(zhi)部(bu)(bu)，將(jiang)經過催芽的(de)棉(mian)(mian)籽(zi)(zi)放(fang)入葵花(hua)(hua)(hua)(hua)(hua)的(de)切口(kou)中，對切口(kou)部(bu)(bu)位捆綁；棉(mian)(mian)籽(zi)(zi)出苗后(hou)，將(jiang)葵花(hua)(hua)(hua)(hua)(hua)在(zai)(zai)切口(kou)上部(bu)(bu)切頭(tou)，形成(cheng)具有葵花(hua)(hua)(hua)(hua)(hua)根莖(jing)(jing)的(de)棉(mian)(mian)花(hua)(hua)(hua)(hua)(hua)苗；育(yu)(yu)苗五(wu)天以(yi)(yi)(yi)后(hou)，按常規方(fang)式(shi)將(jiang)稼接完成(cheng)的(de)棉(mian)(mian)花(hua)(hua)(hua)(hua)(hua)苗向大田移栽，并實施田間管理，直(zhi)至(zhi)(zhi)采摘收(shou)獲。本發(fa)明方(fang)法(fa)可(ke)大幅(fu)度提高棉(mian)(mian)花(hua)(hua)(hua)(hua)(hua)產量、增強(qiang)棉(mian)(mian)花(hua)(hua)(hua)(hua)(hua)抗病蟲害(hai)、抗自然災害(hai)能(neng)力。 

7、棉花的一種扦插繁殖方法 

申(shen)請（專利(li)）號：200510088964.7 

摘要：本發明公開了一種(zhong)(zhong)棉(mian)花的(de)扦(qian)(qian)插(cha)繁(fan)(fan)殖(zhi)方法(fa)。該(gai)方法(fa)包括以下步驟(zou)：1）將(jiang)棉(mian)花枝(zhi)(zhi)(zhi)條(tiao)(tiao)接到(dao)棉(mian)花再(zai)生(sheng)苗(miao)(miao)(miao)(miao)(miao)生(sheng)根(gen)培養(yang)基中(zhong)進(jin)(jin)行(xing)(xing)(xing)擴繁(fan)(fan)生(sheng)根(gen)，或將(jiang)棉(mian)花枝(zhi)(zhi)(zhi)條(tiao)(tiao)的(de)基部先用棉(mian)花枝(zhi)(zhi)(zhi)條(tiao)(tiao)扦(qian)(qian)插(cha)生(sheng)根(gen)劑浸泡20～28小時，再(zai)扦(qian)(qian)插(cha)于棉(mian)花無(wu)土育苗(miao)(miao)(miao)(miao)(miao)基質中(zhong)進(jin)(jin)行(xing)(xing)(xing)生(sheng)根(gen)培養(yang)；2）待棉(mian)花枝(zhi)(zhi)(zhi)條(tiao)(tiao)長出根(gen)系后移栽(zai)，得(de)(de)到(dao)棉(mian)花植株(zhu)。本發明具(ju)有(you)下述優點：一是(shi)可(ke)以利用棉(mian)花枝(zhi)(zhi)(zhi)條(tiao)(tiao)、贅壓進(jin)(jin)行(xing)(xing)(xing)擴繁(fan)(fan)；二是(shi)獲得(de)(de)的(de)快繁(fan)(fan)苗(miao)(miao)(miao)(miao)(miao)根(gen)多、苗(miao)(miao)(miao)(miao)(miao)壯、苗(miao)(miao)(miao)(miao)(miao)病少或無(wu)，取苗(miao)(miao)(miao)(miao)(miao)后根(gen)系保(bao)存完(wan)整；三是(shi)可(ke)實行(xing)(xing)(xing)工廠化育苗(miao)(miao)(miao)(miao)(miao)，育苗(miao)(miao)(miao)(miao)(miao)基質質地無(wu)土，重量輕(qing)，能夠(gou)開展工廠化集中(zhong)保(bao)護育苗(miao)(miao)(miao)(miao)(miao)，且風險小，效果好，節(jie)約了育苗(miao)(miao)(miao)(miao)(miao)面積；四是(shi)可(ke)實行(xing)(xing)(xing)無(wu)載體(ti)單苗(miao)(miao)(miao)(miao)(miao)移栽(zai)，在無(wu)土基質床體(ti)上(shang)取苗(miao)(miao)(miao)(miao)(miao)時幼苗(miao)(miao)(miao)(miao)(miao)無(wu)載體(ti)，此(ci)外(wai)，若(ruo)使用促根(gen)劑，則可(ke)加快移栽(zai)苗(miao)(miao)(miao)(miao)(miao)生(sheng)根(gen)速度(du)，縮(suo)短返苗(miao)(miao)(miao)(miao)(miao)時間，提高成(cheng)苗(miao)(miao)(miao)(miao)(miao)率；五(wu)是(shi)利用枝(zhi)(zhi)(zhi)條(tiao)(tiao)扦(qian)(qian)插(cha)取代棉(mian)種(zhong)(zhong)，解決了雜交棉(mian)制(zhi)種(zhong)(zhong)成(cheng)本高、優良品(pin)種(zhong)(zhong)易退(tui)化的(de)難題。 

8、棉花水培漂浮育苗方法 

申請（專(zhuan)利）號(hao)：200510031883.3 

摘要：本發明涉及一種新型(xing)的棉(mian)花育苗(miao)(miao)方法，用多孔育苗(miao)(miao)盤、基質和營養液水(shui)體苗(miao)(miao)床進行漂浮育苗(miao)(miao)，優良棉(mian)種的出苗(miao)(miao)率達到90％以上，培育出的棉(mian)苗(miao)(miao)根系(xi)發達，生活力強(qiang)，棉(mian)苗(miao)(miao)整齊(qi)一致(zhi)，少病菌株或(huo)無病菌株，無雜草(cao)，成(cheng)苗(miao)(miao)率高(gao)。與之(zhi)相配(pei)套的移栽(zai)(zai)技(ji)術，具有(you)省工，節省成(cheng)本，移栽(zai)(zai)簡(jian)易，取苗(miao)(miao)運苗(miao)(miao)方便，移栽(zai)(zai)成(cheng)活率高(gao)，返苗(miao)(miao)期短，移栽(zai)(zai)時(shi)間靈活性等好特(te)點。